Six tetraploid Alstroemeria clones were micropropagated by rhizome multiplication, a system whereby the shoots were cut off from the rhizome and discarded. In a three-week subculture interval, the average rhizome multiplication rate for all genotypes was 2.3. In one selected genotype the discarded shoots were used to initiate another micropropagation system: the leafy explant micropropagation s...

Introduction Pinus taeda stands for productivity and quality of its timber [1]. Researches using biotechnology are of great importance and have been applied to the improvement of its timber and plantation [2]. The main method of Pinus propagation is by seeds, once the minicuttings depends on the season of the year or depends of juvenile material [3-5]. Thus, researches on micropropagation of Pi...

Conventional propagation of bulbous crops must be supplemented with micropropagation to satisfy the requirements of present-day horticulture with respect to fast production of disease-free, superior starting material. Adequate micropropagation protocols for bulbous crops are therefore a sine qua non. The successive steps in micropropagation of bulbous crops are reviewed: initiation, multiplicat...

Micropropagation is a sophisticated technique for the rapid multiplication of plants. It has a great commercial potential due to the speed of propagation, the high plant quality, and the ability to produce disease-free plants. However, micropropagation is usually done by hand which makes the process cost-intensive and tedious for the workers especially because it requires a sterile work-place. ...

Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and αnaphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm TDZ and 0.1 mg dm NAA. It was noted t...

Galactomannans (GMs) extracted from seeds of a native Brazilian specie designated Cassia fastuosa (cassia) and from Cyamopsis tetragonolobus (guar gum – a commercial GM) were mixed with agar, in the proportion of 3/3 g.L (w/w), and used as a gelling agent in Marubakaido apple rootstock (Malus prunifolia Borkh) micropropagation. The rheological measurements of the gels showed a good interaction ...

Ivan Iliev1, Alena Gajdošová2, Gabriela Libiaková2 and Shri Mohan Jain3∗ 1Faculty of Ecology and Landscape Architecture, University of Forestry, Sofia, Bulgaria 2Institute of Plant Genetics and Biotechnology SAS, Nitra, Slovakia 3Plant Breeding Unit, International Atomic Energy Agency, Laboratories Siebersdorf, Vienna, Austria ∗Current address – Department of Applied Biology, University of Hels...

The use of liquid instead of solid culture medium for the micropropagation of plants offers advantages such as better access to medium components and scalability through possible automation of the processes. The objective of this work was to compare a new temporary immersion bioreactor (TIB) to solid medium culture for the micropropagation of a selection of tree species micropropagated for comm...

A protocol for the micropropagation of Eucalyptus polybractea R.T. Baker (blue mallee) using axillary bud proliferation from lignotuber-derived explants is described. Three different ages of plants were used as explant sources: glasshouse-grown seedlings, field-grown saplings, and coppice of field-grown mature lignotubers. Explants from each source initiated successfully and no significant diff...